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primary antibodies against runx2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology primary antibodies against runx2
    Primary Antibodies Against Runx2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+runx2/RUNX2+Antibody/pm41892959-161-0-13
    Average 96 stars, based on 1561 article reviews
    primary antibodies against runx2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Pharmacological elevation of sphingosine-1-phosphate by S1P lyase inhibition accelerates bone regeneration after post-traumatic osteomyelitis.
    Article Snippet: .. Primary antibodies against Runx2 (M- 70, cat. no. sc- 10758, Santa Cruz Biotechnologies) and CD- 31 (cat. no. 550274, BD Biosciences) were used for immunofluorescent staining. ..

    Article Title: "The role of mitogen-activated protein kinase signaling pathway in bone formation during mid-palatal suture expansion".
    Article Snippet: Purpose: Orthodontic interventions such as maxillary expansion are pivotal in correcting malocclusions; however, the intracellular mechanisms of bone remodeling during this process are not well understood.. This study investigated the role of the mitogen-activated protein kinase (MAPK) pathway in bone remodeling during maxillary expansion and relapse in rats.. Materials and Methods: Thirty male Wistar rats were randomly divided into three groups: Control (Ctrl), Expansion only (EO), and Expansion with MEK inhibitor U0126 (EO + INH).

    Article Title: Pharmacological elevation of sphingosine‐1‐phosphate by S1P lyase inhibition accelerates bone regeneration after post‐traumatic osteomyelitis
    Article Snippet: .. Primary antibodies against Runx2 (M‐70, cat. no. sc‐10758, Santa Cruz Biotechnologies) and CD‐31 (cat. no. 550274, BD Biosciences) were used for immunofluorescent staining. ..

    Immunohistochemical staining:

    Article Title: "The role of mitogen-activated protein kinase signaling pathway in bone formation during mid-palatal suture expansion".
    Article Snippet: Purpose: Orthodontic interventions such as maxillary expansion are pivotal in correcting malocclusions; however, the intracellular mechanisms of bone remodeling during this process are not well understood.. This study investigated the role of the mitogen-activated protein kinase (MAPK) pathway in bone remodeling during maxillary expansion and relapse in rats.. Materials and Methods: Thirty male Wistar rats were randomly divided into three groups: Control (Ctrl), Expansion only (EO), and Expansion with MEK inhibitor U0126 (EO + INH).

    Immunohistochemistry:

    Article Title: "The role of mitogen-activated protein kinase signaling pathway in bone formation during mid-palatal suture expansion".
    Article Snippet: Purpose: Orthodontic interventions such as maxillary expansion are pivotal in correcting malocclusions; however, the intracellular mechanisms of bone remodeling during this process are not well understood.. This study investigated the role of the mitogen-activated protein kinase (MAPK) pathway in bone remodeling during maxillary expansion and relapse in rats.. Materials and Methods: Thirty male Wistar rats were randomly divided into three groups: Control (Ctrl), Expansion only (EO), and Expansion with MEK inhibitor U0126 (EO + INH).



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    Osteogenic differentiation-promoting effect of DBM-DMSN@TPTD composite scaffold on BMSCs. ( A ) ALP staining of BMSCs after 7 days of culture. ( B ) Immunofluorescence staining of <t>RUNX2</t> in BMSCs. ( C ) Immunofluorescence staining of OCN in BMSCs. ( D ) Quantitative analysis of RUNX2 mean fluorescence intensity. ( E ) Quantitative analysis of OCN mean fluorescence intensity. Data are presented as mean ± SD ( n = 3). Comparisons among multiple groups were performed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. ns, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001.
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    primary antibodies against runx2  (Santa Cruz Biotechnology)
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    primary antibody against runx2  (Servicebio Inc)
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    Osteogenic functional analysis of BMSCs in an endothelial co-culture system. ( A ) Wound-healing process of BMSCs and semiquantitative analysis (wound width). ( B ) Quantitative analysis of the wound-healing process at 12 h and 24 h. ( C ) Migration ability of BMSCs evaluated by the Transwell system. ( D ) Quantitative analysis of the migrated BMSCs. ( E ) ALP staining of BMSCs at day 7. ( F ) ARS staining of BMSCs at day 21. ( G ) Quantitative analysis of <t>Runx2</t> fluorescence. ( H ) Representative Runx2 fluorescence image of BMSCs. ( I ) Expression of proteins Runx2 and Col1a1 in BMSCs. ( J , K ) Quantitative analysis of the relative Runx2 and Col1a1 expression. Error bars, mean ± standard deviation; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 ( n = 3).
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    Image Search Results


    Osteogenic differentiation-promoting effect of DBM-DMSN@TPTD composite scaffold on BMSCs. ( A ) ALP staining of BMSCs after 7 days of culture. ( B ) Immunofluorescence staining of RUNX2 in BMSCs. ( C ) Immunofluorescence staining of OCN in BMSCs. ( D ) Quantitative analysis of RUNX2 mean fluorescence intensity. ( E ) Quantitative analysis of OCN mean fluorescence intensity. Data are presented as mean ± SD ( n = 3). Comparisons among multiple groups were performed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. ns, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Pharmaceutics

    Article Title: Dendritic Mesoporous Silica-Modified Decellularized Bone Matrix Scaffold for Sustained Teriparatide Delivery in Bone Defect Repair: Characterization, Drug Release, and In Vitro Biological Performance

    doi: 10.3390/pharmaceutics18091067

    Figure Lengend Snippet: Osteogenic differentiation-promoting effect of DBM-DMSN@TPTD composite scaffold on BMSCs. ( A ) ALP staining of BMSCs after 7 days of culture. ( B ) Immunofluorescence staining of RUNX2 in BMSCs. ( C ) Immunofluorescence staining of OCN in BMSCs. ( D ) Quantitative analysis of RUNX2 mean fluorescence intensity. ( E ) Quantitative analysis of OCN mean fluorescence intensity. Data are presented as mean ± SD ( n = 3). Comparisons among multiple groups were performed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. ns, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Subsequently, the cells were blocked with 5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 30 min. Primary antibodies against RUNX2 (ABclonal Technology Co., Ltd., Wuhan, Hubei, China) and OCN (ABclonal Technology Co., Ltd., Wuhan, Hubei, China) were added and incubated overnight at 4 °C in a humidified chamber.

    Techniques: Staining, Immunofluorescence, Analysis, Fluorescence

    Osteogenic functional analysis of BMSCs in an endothelial co-culture system. ( A ) Wound-healing process of BMSCs and semiquantitative analysis (wound width). ( B ) Quantitative analysis of the wound-healing process at 12 h and 24 h. ( C ) Migration ability of BMSCs evaluated by the Transwell system. ( D ) Quantitative analysis of the migrated BMSCs. ( E ) ALP staining of BMSCs at day 7. ( F ) ARS staining of BMSCs at day 21. ( G ) Quantitative analysis of Runx2 fluorescence. ( H ) Representative Runx2 fluorescence image of BMSCs. ( I ) Expression of proteins Runx2 and Col1a1 in BMSCs. ( J , K ) Quantitative analysis of the relative Runx2 and Col1a1 expression. Error bars, mean ± standard deviation; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 ( n = 3).

    Journal: Polymers

    Article Title: An Electrospun DFO-Loaded Microsphere/SAIB System Orchestrates Angiogenesis–Osteogenesis Coupling via HIF-1α Activation for Vascularized Bone Regeneration

    doi: 10.3390/polym17111538

    Figure Lengend Snippet: Osteogenic functional analysis of BMSCs in an endothelial co-culture system. ( A ) Wound-healing process of BMSCs and semiquantitative analysis (wound width). ( B ) Quantitative analysis of the wound-healing process at 12 h and 24 h. ( C ) Migration ability of BMSCs evaluated by the Transwell system. ( D ) Quantitative analysis of the migrated BMSCs. ( E ) ALP staining of BMSCs at day 7. ( F ) ARS staining of BMSCs at day 21. ( G ) Quantitative analysis of Runx2 fluorescence. ( H ) Representative Runx2 fluorescence image of BMSCs. ( I ) Expression of proteins Runx2 and Col1a1 in BMSCs. ( J , K ) Quantitative analysis of the relative Runx2 and Col1a1 expression. Error bars, mean ± standard deviation; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 ( n = 3).

    Article Snippet: A primary antibody against Runx2 (rabbit monoclonal, 1:200; Servicebio, Wuhan, China) was applied overnight at 4 °C, followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (2 h, RT, 1:500; Servicebio, Wuhan, China).

    Techniques: Functional Assay, Co-Culture Assay, Migration, Staining, Fluorescence, Expressing, Standard Deviation

    Bone regeneration efficacy in calvarial defects. ( A ) Establishment of a rat calvarial-defect model (defect size = 5 mm). ( B ) Rat skull 3D rendering at 8 weeks. ( C ) H&E staining and Masson‘s trichrome staining at 8 weeks (NB: new bone; DM: defect margin; OB: osteoblast; CT: connective tissue; BV: blood vessels). ( D ) Paired difference in BV/TV. ( E ) Paired difference in relative new-bone area. ( F ) Paired difference in BMD. ( G ) Immunohistochemical staining of Runx2 and Col1a1. ( H ) Quantitative evaluation of the expression of Runx2 and Col1a1. Error bars, mean ± standard deviation; ** p < 0.01; **** p < 0.0001 ( n = 5).

    Journal: Polymers

    Article Title: An Electrospun DFO-Loaded Microsphere/SAIB System Orchestrates Angiogenesis–Osteogenesis Coupling via HIF-1α Activation for Vascularized Bone Regeneration

    doi: 10.3390/polym17111538

    Figure Lengend Snippet: Bone regeneration efficacy in calvarial defects. ( A ) Establishment of a rat calvarial-defect model (defect size = 5 mm). ( B ) Rat skull 3D rendering at 8 weeks. ( C ) H&E staining and Masson‘s trichrome staining at 8 weeks (NB: new bone; DM: defect margin; OB: osteoblast; CT: connective tissue; BV: blood vessels). ( D ) Paired difference in BV/TV. ( E ) Paired difference in relative new-bone area. ( F ) Paired difference in BMD. ( G ) Immunohistochemical staining of Runx2 and Col1a1. ( H ) Quantitative evaluation of the expression of Runx2 and Col1a1. Error bars, mean ± standard deviation; ** p < 0.01; **** p < 0.0001 ( n = 5).

    Article Snippet: A primary antibody against Runx2 (rabbit monoclonal, 1:200; Servicebio, Wuhan, China) was applied overnight at 4 °C, followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (2 h, RT, 1:500; Servicebio, Wuhan, China).

    Techniques: Staining, Immunohistochemical staining, Expressing, Standard Deviation